Human glial fibrillary acidic protein (GFAP), technical only
Use
GFAP (Glial Fibrillary Acidic Protein) is an intermediate filament protein predominantly expressed in astrocytes and other glial cells in the central nervous system. In pathology, GFAP is used to identify astrocytic tumors and to distinguish them from other types of central nervous system neoplasms. Its expression also aids in the diagnosis of reactive gliosis and Alexander disease, where abnormal GFAP accumulation is observed. Technical component only. Pathologist read not included.
Special Instructions
Fresh Tissues: Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Samples must be tested 3 months after placing in ethanol. Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
7 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED). Samples may be transferred to 70% alcohol for transportation purposes after fixation in 10% NBF or B plus.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |