Human herpesvirus type 8 (HHV-8), technical only
Use
HHV-8 (Human Herpesvirus 8) is a key pathogen in the development of Kaposi's sarcoma, primary effusion lymphoma, and multicentric Castleman's disease. The immunohistochemistry test is used to identify infected cells in tissue samples, assisting in the diagnosis of KSHV-related malignancies. This test provides the technical component only and does not include a pathologist read.
Special Instructions
Fresh Tissues should be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer-term storage if needed. Unstained slides require positively charged glass slides to prevent background staining and tissue loss, and should be left unbaked.
Limitations
The test only includes the technical component and does not cover the pathologist's interpretation of results. It requires specific specimen preparations, including fixation in 10% neutral buffered formalin or B plus. Use of other fixatives will lead to specimen rejection.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |