IGH 14q32 Rearrangement, paraffin
Use
FISH testing on paraffin-embedded cells for detection of IGH (14q32) gene rearrangement. IGH is frequently rearranged in a variety of neoplasms, including B-cell lymphoma, lymphoproliferative disorder, CLL, and myeloid neoplasms.
Special Instructions
Tissues should be fixed in 10% neutral buffered formalin (NBF). Tissues preserved in B+ fixative or decalcified are usually not suitable for FISH.
Limitations
Assay requires 3 unstained slides minimum (two for each probe) and one H&E stained slide. If unstained slides are sent, cut FFPE tissue sections at 3-5 μm thickness, mounted on positively charged slides.
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
5 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
3 slides minimum
Container
positively charged slides
Collection Instructions
Cut FFPE tissue sections at 3-5 μm thickness, mounted on positively charged slides.
Causes for Rejection
Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Stable indefinitely |