IGH::BCL2; t(14;18), paraffin
Use
FISH testing on paraffin-embedded cells for detection of IGH::BCL2 gene fusion. This fusion, associated with t(14;18), is frequently observed in B-cell malignancies, including follicular lymphoma and diffuse large B-cell lymphoma (DLBCL). Often ordered as part of the FP BURKITT Panel test.
Special Instructions
Tissues should be fixed in 10% neutral buffered formalin (NBF). Tissues preserved in B+ fixative or decalcified are usually not suitable for FISH. Assay requires 3 unstained slides minimum (two for each probe) and one H&E stained slide.
Limitations
Improper fixation or unsuitable preservation such as in B+ fixative or decalcified tissues can render samples unsuitable for FISH analysis. Extreme temperatures during sample handling can compromise test quality.
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
5 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
FFPE Tissue Block or Unstained FFPE tissue slides
Collection Instructions
Cut FFPE tissue sections at 3-5 μm thickness, mounted on positively charged slides.
Storage Instructions
15°C to 25°C
Causes for Rejection
Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Stable indefinitely |