IGH::CCND1 (BCL1); t(11;14), paraffin
Use
FISH testing on paraffin-embedded cells for detection of IGH::CCND1 gene fusion. This fusion, associated with t(11;14), is observed in > 90% of mantle cell lymphomas and a subset of plasma cell neoplasms, follicular lymphoma, endometrial adenocarcinoma, and prolymphocytic leukemia.
Special Instructions
Tissues should be fixed in 10% neutral buffered formalin (NBF). Tissues preserved in B+ fixative or decalcified are usually not suitable for FISH. Assay requires 3 unstained slides minimum (two for each probe) and one H&E stained slide. If unstained slides are sent, cut FFPE tissue sections at 3-5 μm thickness, mounted on positively charged slides.
Limitations
Improper fixative, such as B+ fixative or decalcified tissues, may render the specimen unsuitable for FISH analysis. The test is limited by the quality and amount of tissue available. Specimens exposed to extreme temperatures or with insufficient tissue may be rejected.
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
5 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Unstained FFPE tissue: 5 slides preferred (3 slides minimum) cut at 3-5 μM and mounted on positively charged slides.
Storage Instructions
Store at 15°C to 25°C. Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen. Do not freeze.
Causes for Rejection
Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Stable indefinitely |