IGH::MYC; t(8;14), paraffin
Use
FISH testing on paraffin-embedded cells for detection of IGH::c-MYC gene fusion associated with t(8;14). Depending on clinical, immunophenotypic, and morphologic context, IGH::c-MYC fusion can support a diagnosis of Burkitt lymphoma. However, this fusion may occur in the progression of non-Burkitt lymphomas and plasma cell neoplasms. Often ordered as part of the FP BURKITT Panel test.
Special Instructions
Tissues should be fixed in 10% neutral buffered formalin (NBF). Tissues preserved in B+ fixative or decalcified are usually not suitable for FISH. Assay requires 3 unstained slides minimum (two for each probe) and one H&E stained slide. If unstained slides are sent, cut FFPE tissue sections at 3-5 μm thickness, mounted on positively charged slides.
Limitations
The assay performance depends on the quality and fixation of the tissue sample. Inappropriate fixation methods or decalcified tissues may not be suitable for analysis via FISH.
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
5 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Storage Instructions
15°C to 25°C
Causes for Rejection
Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Stable indefinitely |