Periodic Acid-Schiff Stain with Diastase (Fungus)
Use
PAS-D fungus special stain is a modification of the PAS technique used to identify fungal organisms, particularly those with a glycogen-rich cell wall, such as Candida species. This stain highlights fungal elements in magenta, while diastase treatment breaks down glycogen, aiding in the distinction between fungal structures and glycogen deposits.
Special Instructions
Fresh Tissue: Specimens may be fixed in formalin for 6-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. This is NOT preferred. Samples should be tested 3 months after placing in ethanol. Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected. Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements.
Methodology
Other (Anatomic Pathology)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED). Specimens may be transferred to 70% alcohol for transportation purposes after fixation in 10% NBF or B plus.
Storage Instructions
Store at 15°C to 25°C. Fresh tissue tissues ≤ 72 Hours.
Causes for Rejection
All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |