Periodic Acid-Schiff Stain with Diastase (Fungus), technical only
Use
PAS-D fungus special stain is a modification of the PAS technique used to identify fungal organisms, particularly those with a glycogen-rich cell wall, such as Candida species. This stain highlights fungal elements in magenta, while diastase treatment breaks down glycogen, aiding in the distinction between fungal structures and glycogen deposits. Technical component only. Pathologist read not included.
Special Instructions
Fresh Tissue: Specimens may be fixed in formalin for 6-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. This is NOT preferred. Samples should be tested 3 months after placing in ethanol. Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected. Indefinite stability for FFPE tissues and unstained slides, while fresh tissue has a stability of ≤ 72 hours.
Methodology
Other (Anatomic Pathology)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
FFPE Block, Formalin Fixed
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
15°C to 25°C
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinite |