ROS1 6q22.1 Rearrangement, paraffin
Use
FISH testing on paraffin-embedded cells for detection of ROS1 (6q22.1) gene rearrangement. ROS1 rearrangements occur in a subset of non-small cell lung cancers (NSCLCs) and, less frequently, in glioma. This test is important for identifying ROS1 rearrangements, which can be pivotal in the diagnosis and treatment planning for patients with these types of cancers. It is often ordered alongside other tests such as FP ALK to provide a comprehensive analysis.
Special Instructions
Tissues should be fixed in 10% neutral buffered formalin (NBF). Tissues preserved in B+ fixative or decalcified are usually not suitable for FISH. Assay requires 3 unstained slides minimum (two for each probe) and one H&E stained slide. If unstained slides are sent, cut FFPE tissue sections at 3-5 μm thickness, mounted on positively charged slides.
Limitations
The test may not be suitable for tissues preserved in B+ fixative or those that are decalcified. Specimens must be handled properly to ensure accurate results, including avoiding exposure to extreme temperatures. Insufficient or improperly fixed tissue can lead to rejection of the specimen. The accuracy of the test results can also be affected by the condition and handling of the tissue.
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
5 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
3 slides minimum
Container
Positively charged slides
Collection Instructions
Cut FFPE tissue sections at 3-5 μm thickness, mounted on positively charged slides. Ensure proper fixation with 10% neutral buffered formalin.
Storage Instructions
Store at 15°C to 25°C. Use ambient shipping conditions with a refrigerated gel pack (not frozen) to protect from extreme temperatures.
Causes for Rejection
Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Stable indefinitely |