S100 Red, technical only
Use
S100 is a calcium-binding protein widely expressed in various cell types, including those of neural, glial, and mesenchymal origin. In pathology, S100 is commonly used to help identify tumors of neural or melanocytic origin, such as schwannomas, melanomas, and dermatofibrosarcoma protuberans. This assay is performed with RED chromogen.
Special Instructions
Technical component only. Pathologist read not included.
Limitations
Samples must be tested 3 months after placing in ethanol. Fresh tissues must be fixed in formalin and then can be transferred to ethanol. Unstained slides must be positively charged to prevent background staining and tissue loss.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED). Specimens may be transferred to 70% alcohol for transportation after fixation.
Storage Instructions
Specimens should be stored at 15°C to 25°C.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |