T-cell Subsets, CD4:CD8 Ratio and Count
Use
This test is used for the analysis of T-cell subsets, specifically providing information on CD3, CD4, and CD8 cells. It also includes the calculation of the CD4:CD8 ratio and count. The test is essential for understanding immune function and is particularly relevant in the context of primary immunodeficiencies and HIV, providing insights into immune dysfunction.
Special Instructions
Not provided.
Limitations
Specimens must be handled carefully to avoid rejection. Common causes for rejection include incorrect temperature storage, inappropriate anticoagulant use, inadequate cell count, hemolysis, and clotting. It's important to adhere to specified storage and shipping requirements to maintain specimen viability.
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
CD3
ProteinCD4
ProteinCD8
Protein
Result Turnaround Time
3 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
4 mL
Minimum Volume
0.5 mL with 107 cells
Container
NaHep preferred (EDTA accepted)
Storage Instructions
2°C to 8°C
Causes for Rejection
Specimens stored at incorrect temperature; Non-viable specimens; Specimens in inappropriate anticoagulant; Too few cells; Hemolysis; Specimen clotted
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | Specimen stable for 48 hours at 4°C |