CD20 Immunohistochemistry (IHC)
Use
CD20 is expressed on mature B‑cells (after CD19 and CD10 expression and before CD21/22 and surface immunoglobulin) and on follicular dendritic cells. In disease, CD20 is positive in most B‑cell lymphomas (including some B‑ALL/LBL), lymphocyte predominant Hodgkin lymphoma, and dimly expressed in benign and neoplastic T‑cells. CD20 expression may be lost in rituximab‑treated B‑cell lymphomas.
Special Instructions
Use a formalin‑fixed, paraffin‑embedded (FFPE) tissue block as preferred specimen. Alternatively, submit one unbaked, unstained H&E slide (required) plus two to three positively charged unstained slides, all cut at 4‑5 microns. Block and slide identifiers must match specimen ID and requisition. For transport, use a cold pack not in direct contact with specimen; New York State clients should include date and time of collection.
Limitations
Not provided.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
24 hours
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Formalin‑fixed, paraffin‑embedded tissue block preferred; or one unbaked unstained H&E slide (required) plus two to three positively charged unstained slides, all cut at 4‑5 microns. Block and slide identifiers must match.