High-Grade B-Cell Lymphoma Reflex FISH Panel
Use
The High-Grade B-Cell Lymphoma Reflex Panel differentiates double-hit or triple-hit lymphomas (which have MYC rearrangements together with BCL2 and/or BCL6 rearrangements) from Burkitt lymphoma or diffuse large B-cell lymphoma. Double-hit and triple-hit lymphomas are difficult to classify morphologically without aid of cytogenetics/FISH or IHC, and are associated with an aggressive course. Testing is indicated when B-cell lymphoma patients experience transformation, relapse, or refractory disease.
Special Instructions
This reflex panel may be considered a cost-effective alternative to the High-Grade/Large B-Cell Lymphoma FISH Panel when clinical circumstances allow an additional few days for reflex testing if MYC is rearranged.
Limitations
IGK/MYC t(2;8), IGL/MYC t(8;22) and BCL6/MYC t(3;8) studies are useful to further subclassify lymphomas that are positive for MYC gene rearrangements, but negative for the most common IGH/MYC translocation. In addition, when both MYC and BCL6 gene rearrangements are present, but no IGH/MYC translocation is identified, these studies may help to differentiate between the double-hit/triple-hit lymphomas (D/T-HL), which have a poor prognosis, and DLBCL with BCL6/IGH translocation, representing a subset of GC B-cell lymphomas distinct from conventional D/T-HL and with better prognosis (so-called "pseudo-double-hit lymphoma").
New York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
3-5 days
Related Documents
For more information, please review the documents below
Specimen
Bone Marrow
Volume
1-2 mL
Minimum Volume
Not provided
Container
sodium heparin tube
Storage Instructions
Use cold pack for transport, making sure cold pack is not in direct contact with specimen. For fresh samples: refrigerate before shipping and ship same day as drawn whenever possible. Do not freeze.