NeoTYPE® Follicular Lymphoma Profile
Also known as: Follicular Lymphoma Profile
Use
Follicular lymphoma (FL) is among the most common non-Hodgkin lymphomas worldwide. FL is a fairly heterogeneous disease, with many patients achieving long-term remission and others who will have a remarkably poor outcome. Approximately 90% of FL cases harbor translocations involving BCL2, and the majority of these FL patients will also have one or more additional recurrent gene mutations. Variant follicular lymphomas such as pediatric-type FL, diffuse FL, and primary cutaneous follicle center lymphoma, have been characterized by the absence of BCL2, BCL6, and IRF4 gene rearrangements. Recent NGS studies have identified frequent/recurrent gene mutations and rearrangements in follicular lymphoma variants; for example, 70% of pediatric-type FL will have mutations in TNFRSF14 and/or MAP2K1, compared to 9% of BCL2-negative FL in adults. Genomic profiling in follicular lymphoma will not only provide diagnostic value, but may also suggest prognostic and therapeutic relevance.
Special Instructions
Test Customization: FISH components may be ordered separately as Tech-Only by pathology clients. Storage and Transportation: Use cold pack for transport, making sure cold pack is not in direct contact with specimen. Ship same day as drawn whenever possible. Please select Extract & Hold - TNA if specimen hold service is desired.
Limitations
Do not use zinc fixatives. If submitting slides, please use positively-charged slides and 10% NBF fixative. Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition. Do not use: Mercury fixatives (B5). Highly acidic or prolonged decalcification processes will not yield sufficient nucleic acid to accurately perform molecular studies.
Methodology
NGS
Biomarkers
Result Turnaround Time
5-14 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Paraffin block or positively-charged slides
Collection Instructions
H&E slide: Required, plus paraffin block. Note on FFPE: Paraffin block is preferred.
Storage Instructions
Use cold pack for transport, making sure cold pack is not in direct contact with specimen.
Causes for Rejection
Do not use zinc fixatives. Mercury fixatives (B5) are also unsuitable. Highly acidic or prolonged decalcification processes will not yield sufficient nucleic acid.