Bacterial 16S rDNA Sequencing
Use
Bacterial 16S rDNA sequencing is a genotypic test used as an adjunct to the phenotypic characteristics of an unknown bacterial isolate to determine the genus and/or genus and species of the isolate. It is especially useful in identifying unusual or emerging pathogens.
Special Instructions
Submit a pure bacterial isolate on appropriate culture medium (agar slant) in a double‑walled container; subculture to appropriate slant before submission. Avoid submission of frozen specimens or mixed cultures.
Limitations
Mixed cultures and frozen specimens are not acceptable and may lead to test failure. Viability of the isolate must be maintained; frozen submissions are unacceptable.
Methodology
Sanger
Biomarkers
Result Turnaround Time
9 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Unknown)
Volume
Not provided
Minimum Volume
Not provided
Container
agar slant, in double‑walled container
Collection Instructions
Subculture isolate to the appropriate slant and submit in double‑walled container
Causes for Rejection
Mixed culture • Received frozen
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Determined by viability |
| Refrigerated | Determined by viability |
| Frozen | Unacceptable |