Borrelia Species DNA, Real‑Time PCR, with Reflexes, Blood
Also known as: Lyme, Borreliosis, Tick borne
Use
This PCR assay detects the presence of Borrelia species DNA during infection prior to the development of antibodies. Amplification of Borrelia genomic DNA from blood can support the clinical diagnosis of Borreliosis. A negative result does not rule out Borrelia infection because spirochetemia in blood may only be present for a limited time; appropriate serology (antibody) testing may be clinically warranted. Additionally, if Borrelia species DNA is detected, reflex testing is performed to differentiate B. burgdorferi (Lyme disease) and B. miyamotoi (tick‑borne relapsing fever); detection of Borrelia species DNA with negative results for both specific assays may indicate infection by other Borrelia species.
Special Instructions
Not provided.
Limitations
Not provided.
Methodology
PCR-based (PCR)
Biomarkers
Borrelia burgdorferi
MicroorganismBorrelia miyamotoi
MicroorganismBorrelia species
Microorganism
Result Turnaround Time
2-3 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
3 mL
Minimum Volume
0.5 mL
Container
EDTA (lavender‑top) or ACD (yellow‑top) tube
Causes for Rejection
Lithium heparin (green‑top) tube; sodium heparin (green‑top) tube
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 7 days |
| Frozen | Unacceptable |
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