Chlamydia trachomatis RNA, TMA, Rectal
Use
This test utilizes transcription-mediated amplification (TMA), a nucleic acid amplification test (NAAT) method, to detect Chlamydia trachomatis RNA in rectal swab specimens. It is intended for extragenital (rectal) screening in individuals with suspected exposure or symptoms, based on CDC recommendations for testing sites directed by sexual history or symptoms. Repeat testing of a positive sample using the same methodology is not typically required unless in special circumstances where confirmatory testing is clinically indicated. ([questdiagnostics.com](https://www.questdiagnostics.com/healthcare-professionals/clinical-education-center/faq/faq154?utm_source=openai))
Special Instructions
Not provided.
Limitations
Limitations include that confirmatory repeat testing using the same methodology is not recommended except in rare cases of potential adverse medical, social, or psychological consequences or low prevalence settings. In such cases, alternative-target TMA tests for rectal specimens are available to confirm positive results. ([questdiagnostics.com](https://www.questdiagnostics.com/healthcare-professionals/clinical-education-center/faq/faq154?utm_source=openai))
Methodology
PCR-based (NAAT (TMA))
Biomarkers
Result Turnaround Time
Not provided.
Related Documents
For more information, please review the documents below
Specimen
Rectal Swab
Volume
Not provided
Minimum Volume
Not provided
Container
APTIMA® Transport Tube (e.g., Combo 2 or Multitest transport tube); alternative: APTIMA® Unisex Swab Collection Kit
Collection Instructions
Rectal swab collected and submitted in APTIMA® transport tube. Preferred: APTIMA® Combo 2 Transport Media or APTIMA® Multitest transport tube. Acceptable: APTIMA® Unisex Swab Collection Kit. Swab inserted approximately 3–5 cm into rectum, rotated against rectal wall ≥3 times; avoid gross fecal contamination; break shaft, recap, label, and ship. ([intellitestmanager.questdiagnostics.com](https://intellitestmanager.questdiagnostics.com/home/output/219594?utm_source=openai))
Causes for Rejection
Grossly contaminated swab (e.g., feces) should be discarded and recollected. ([intellitestmanager.questdiagnostics.com](https://intellitestmanager.questdiagnostics.com/home/output/250278?utm_source=openai))
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