Cyclosporine A, Trough, LCMSMS, Blood
Use
Cyclosporine is a commonly used immunosuppressive drug in patients receiving transplants. LC/MS/MS methods have higher specificity for the parent compound than immunoassay. Therapeutic drug monitoring is useful to optimize dose and avoid toxicity. High cyclosporine levels can lead to nephrotoxicity; low levels can lead to organ rejection following transplant. Peak concentrations are reached at approximately 3.5 hours after oral dosage, with elimination half‑life of 10–27 hours.
Special Instructions
Preferred specimen: 5 mL (minimum 2 mL) whole blood collected in an EDTA (lavender‑top) tube. Acceptable alternative: whole blood in sodium heparin (green‑top) tube. Do not use gel barrier / serum separator tubes. Optimum collection is 1 hour before next dose (trough). Reject if clotted. Transport at room temperature. Setup Mon‑Sat; report available in 1–3 days.
Limitations
Not provided.
Methodology
Mass Spectrometry
Biomarkers
LOINC Codes
- 53828-0
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
5 mL
Minimum Volume
2 mL
Container
EDTA (lavender‑top) tube
Collection Instructions
Collect trough specimen approximately 1 hour before next dose
Causes for Rejection
Clotted
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 6 days |
| Frozen | Unacceptable |