dRVVT Screen with Reflex to dRVVT Confirm and dRVVT 1:1 Mix

Also known as: Dilute Russell Viper Venom

Clinical Use

Use

The dilute Russell Viper Venom Time (dRVVT) integrated test is a sensitive method for the detection of Lupus Anticoagulants (LA), which are autoantibodies targeting protein‑phospholipid complexes. In some patients, a confirmed positive result is associated with increased thrombotic risk and may be found in conditions such as recurrent fetal loss. International consensus guidelines recommend using two different screening assays (e.g., PTT‑LA and dRVVT) to increase detection sensitivity, though here the focus is on dRVVT screening followed by confirmatory and mixing studies as reflexes when indicated.

Special Instructions

Not provided.

Limitations

Residual platelets in frozen‑thawed plasma can decrease sensitivity and specificity of testing (risking false‑negative results); specimens to be frozen must be centrifuged twice to remove as many platelets as possible before freezing. The dRVVT test will not detect all lupus anticoagulants, and certain anticoagulant therapies (e.g., warfarin, heparin, direct thrombin or factor Xa inhibitors) may interfere with assay performance, potentially leading to false positives. Interpretation should account for clinical context and potential interfering substances.

Test Details

Methodology

Other

Biomarkers

dRVVT Screen / Confirm / 1:1 Mix Reflex Workflow

Result Turnaround Time

Not provided.

Specimen

Plasma

Volume

1 mL (0.5 mL minimum)

Minimum Volume

Not provided

Container

3.2% sodium citrate (light blue‑top) tube

Collection Instructions

Draw blood in a light blue‑top tube containing 3.2% sodium citrate, mix gently by inverting 3‑4 times, centrifuge 15 minutes at 1500 g within one hour of collection, remove plasma avoiding the WBC/platelet buffy layer, place into a plastic vial.