dRVVT Screen with Reflex to dRVVT Confirm and dRVVT 1:1 Mix
Also known as: Dilute Russell Viper Venom
Use
The dilute Russell Viper Venom Time (dRVVT) integrated test is a sensitive method for the detection of Lupus Anticoagulants (LA), which are autoantibodies targeting protein‑phospholipid complexes. In some patients, a confirmed positive result is associated with increased thrombotic risk and may be found in conditions such as recurrent fetal loss. International consensus guidelines recommend using two different screening assays (e.g., PTT‑LA and dRVVT) to increase detection sensitivity, though here the focus is on dRVVT screening followed by confirmatory and mixing studies as reflexes when indicated.
Special Instructions
Not provided.
Limitations
Residual platelets in frozen‑thawed plasma can decrease sensitivity and specificity of testing (risking false‑negative results); specimens to be frozen must be centrifuged twice to remove as many platelets as possible before freezing. The dRVVT test will not detect all lupus anticoagulants, and certain anticoagulant therapies (e.g., warfarin, heparin, direct thrombin or factor Xa inhibitors) may interfere with assay performance, potentially leading to false positives. Interpretation should account for clinical context and potential interfering substances.
Methodology
Other
Biomarkers
Result Turnaround Time
Not provided.
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
1 mL (0.5 mL minimum)
Minimum Volume
Not provided
Container
3.2% sodium citrate (light blue‑top) tube
Collection Instructions
Draw blood in a light blue‑top tube containing 3.2% sodium citrate, mix gently by inverting 3‑4 times, centrifuge 15 minutes at 1500 g within one hour of collection, remove plasma avoiding the WBC/platelet buffy layer, place into a plastic vial.
Storage Instructions
Frozen
Stability Requirements
| Temperature | Period |
|---|---|
| Frozen | 30 days |