Glucose, Gestational Screen (50g), 140 Cutoff
Also known as: Glucose, Gestational Screen, Gestational, Glucose
Use
This test is the first‐step screening test for gestational diabetes mellitus (GDM), typically performed between 24 and 28 weeks of pregnancy. It involves administration of a 50 g glucose load, with measurement of blood glucose one hour later. A result ≥ 140 mg/dL indicates abnormal glycemic response and the need for a diagnostic glucose tolerance test (e.g., 3‑hour OGTT)([questdiagnostics.com](https://www.questdiagnostics.com/healthcare-professionals/about-our-tests/womens-health/pregnancy-and-fertility/pregnant-patients/testing-for-each-trimester?utm_source=openai)).
Special Instructions
Fasting is not required. The test is performed one hour after ingestion of a 50 g glucose beverage. It is supported by ACOG and ADA as the first step of the two‑step GDM evaluation([questdiagnostics.com](https://www.questdiagnostics.com/healthcare-professionals/about-our-tests/womens-health/pregnancy-and-fertility/pregnant-patients/testing-for-each-trimester?utm_source=openai)).
Limitations
This screening test does not diagnose GDM—it identifies individuals at risk. False negatives may occur, particularly if performed under non‑standard conditions. Sensitivity and specificity vary; lower glucose cutoffs increase sensitivity but reduce specificity. Interpretation should be in clinical context, and abnormal results require follow‑up testing([uspreventiveservicestaskforce.org](https://www.uspreventiveservicestaskforce.org/uspstf/recommendation/gestational-diabetes-screening?utm_source=openai)).
Methodology
Automated Analyzer (Clinical Chemistry)
Biomarkers
Result Turnaround Time
Not provided.
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
Not provided
Minimum Volume
Not provided
Container
Fluoride/oxalate (gray‑top) tube preferred; whole blood in fluoride/oxalate, green‑top or SST acceptable
Collection Instructions
One‑hour timed specimen after 50 g glucose ingestion; fasting not required; remove plasma/serum from cells immediately
Storage Instructions
Serum/plasma: room temperature; whole blood: refrigerated
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days (sodium fluoride plasma); serum: 4 days |
| Refrigerated | 7 days (plasma and serum) |
| Frozen | 28 days (serum and plasma) |
