HIV‑1 Resistance, Proviral DNA (RT, PI Inhibitors)
Also known as: Archived DNA, Undetected viral load, Cell-associated Viral DNA, Archived resistance, Resistance Analysis
Use
This test utilizes PCR and next‑generation (ultra‑deep) sequencing of proviral DNA to detect drug resistance mutations in the HIV‑1 protease and reverse transcriptase genes. It assists clinicians in modifying treatment regimens for virologically suppressed patients or those with low viral load for whom an RNA-based resistance test was unsuccessful. The test amplifies the pol gene regions (protease codons 1‑99 and reverse transcriptase codons 1‑560) from EDTA whole blood, enabling detection of archived resistance mutations to NRTI, NNRTI, and PI drug classes. ([jdos.nicholsinstitute.com](https://jdos.nicholsinstitute.com/dos/nmmcpathology/test/573433?utm_source=openai))
Special Instructions
Not provided.
Limitations
The PCR primers effectively amplify HIV‑1 subtype B and major non‑B subtypes (A, C, BF, D, AE, AG, and H), but amplification may fail for uncommon non‑B subtypes or strains. Clinical performance data remain limited; archived resistance mutations may not encompass all prior plasma RNA findings, and some resistance patterns may no longer be detectable in proviral DNA. ([questdiagnostics.com](https://www.questdiagnostics.com/healthcare-professionals/clinical-education-center/faq/faq198?utm_source=openai))
Methodology
NGS (Targeted)
Biomarkers
Result Turnaround Time
6-8 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
2 mL
Minimum Volume
0.6 mL
Container
lavender‑top EDTA tube
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