HIV‑1 Resistance, Proviral DNA (RT, PI Inhibitors)

Casandra

Casandra Test Code QD01082Version 1 (DRAFT)

Performing Lab

Lab Quest DiagnosticsLab Test ID 94808CPT Codes 87900, 87901

HIV‑1 Resistance, Proviral DNA (RT, PI Inhibitors)

Also known as: Archived DNA, Undetected viral load, Cell-associated Viral DNA, Archived resistance, Resistance Analysis

Clinical Use

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Use

This test utilizes PCR and next‑generation (ultra‑deep) sequencing of proviral DNA to detect drug resistance mutations in the HIV‑1 protease and reverse transcriptase genes. It assists clinicians in modifying treatment regimens for virologically suppressed patients or those with low viral load for whom an RNA-based resistance test was unsuccessful. The test amplifies the pol gene regions (protease codons 1‑99 and reverse transcriptase codons 1‑560) from EDTA whole blood, enabling detection of archived resistance mutations to NRTI, NNRTI, and PI drug classes. ([jdos.nicholsinstitute.com](https://jdos.nicholsinstitute.com/dos/nmmcpathology/test/573433?utm_source=openai))

Special Instructions

Not provided.

Limitations

The PCR primers effectively amplify HIV‑1 subtype B and major non‑B subtypes (A, C, BF, D, AE, AG, and H), but amplification may fail for uncommon non‑B subtypes or strains. Clinical performance data remain limited; archived resistance mutations may not encompass all prior plasma RNA findings, and some resistance patterns may no longer be detectable in proviral DNA. ([questdiagnostics.com](https://www.questdiagnostics.com/healthcare-professionals/clinical-education-center/faq/faq198?utm_source=openai))

Test Details