HIV-1 RNA, Quantitative, Real‑Time PCR with Reflex to Genotype (RTI, PI, Integrase)
Also known as: Human Immunodeficiency Virus
Use
Used to monitor HIV‑1 viral load in patients undergoing antiretroviral therapy, this test measures quantitative levels of HIV‑1 RNA. If viral load exceeds thresholds indicating virologic failure, the assay reflexively triggers genotypic resistance testing in reverse transcriptase inhibitor, protease inhibitor, and integrase regions to aid in treatment management and detection of drug resistance.
Special Instructions
Reflex testing to genotype (RTI, PI, Integrase) is automatically performed when HIV‑1 RNA level exceeds the specified threshold for virologic failure. Additional charges apply for genotype testing. Ensure proper plasma separation and transport per specimen requirements.
Limitations
Genotyping requires a minimum viral load (recommended 500–1,000 copies/mL); undetectable or low-level viral loads may not produce reliable genotype results. RNA degradation due to improper collection, storage, or transport can lead to failure to amplify, reflected as “Not Detected.”
Methodology
PCR‑based (RT‑PCR)
Biomarkers
LOINC Codes
- 20447-9
Result Turnaround Time
2-4 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
7 mL
Minimum Volume
3.7 mL
Container
EDTA (lavender‑top) tube
Collection Instructions
Do not freeze whole blood in primary tube. Fresh whole blood may be stored at 2‑25 °C for up to 24 hours prior to centrifugation. Separate plasma within 24 hours.
Causes for Rejection
Serum; heparinized plasma; specimens sent at ambient temperature; non‑centrifuged specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 6 days refrigerated |
| Frozen | 30 days frozen |