HLA-B Typing, High Resolution
Use
HLA‑B*5701 typing can be used for genetic risk stratification prior to initiation of abacavir therapy to assess risk of hypersensitivity reactions; abacavir‑associated hypersensitivity is strongly associated with presence of the HLA‑B*5701 allele.
Special Instructions
Not provided.
Limitations
A negative result does not rule out the risk for abacavir hypersensitivity reaction and clinical vigilance must be maintained regardless of result.
Methodology
PCR-based
Biomarkers
Result Turnaround Time
Not provided.
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
5 mL (preferred), minimum 3 mL
Minimum Volume
3 mL
Container
EDTA (lavender‑top) tube; also acceptable: sodium heparin (green‑top), EDTA pink‑top, EDTA royal blue‑top, sodium citrate (light blue‑top)
Collection Instructions
A separate EDTA (lavender‑top) tube must be submitted for all HLA testing. If submitted with any other test requiring an EDTA tube, please submit a separate tube for this test. Do not open tube.
Causes for Rejection
Received frozen; Clotted
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 7 days |
| Frozen | Unacceptable |