Use

High‑sensitivity quantitative flow cytometry assay for diagnosis and follow‑up of paroxysmal nocturnal hemoglobinuria (PNH). It evaluates glycophosphatidylinositol (GPI)‑linked antigens such as CD59 on erythrocytes and uses FLAER to assess granulocytes and monocytes, enabling detection of PNH clones down to 0.01%. Monitoring clone size is especially useful for patients initiating treatment or with myelodysplastic syndromes or aplastic anemia, where small clones may be clinically significant. Peripheral blood is the only recommended specimen, as bone marrow specimens are not suitable.