Paroxysmal Nocturnal Hemoglobinuria (PNH) with FLAER (High Sensitivity)
Also known as: Fluorescent Proaerolysin Variant (FLAER), PI Linked Antigen, Blood, Flow, GPI-Linked Proteins
Use
High‑sensitivity quantitative flow cytometry assay for diagnosis and follow‑up of paroxysmal nocturnal hemoglobinuria (PNH). It evaluates glycophosphatidylinositol (GPI)‑linked antigens such as CD59 on erythrocytes and uses FLAER to assess granulocytes and monocytes, enabling detection of PNH clones down to 0.01%. Monitoring clone size is especially useful for patients initiating treatment or with myelodysplastic syndromes or aplastic anemia, where small clones may be clinically significant. Peripheral blood is the only recommended specimen, as bone marrow specimens are not suitable.
Special Instructions
Not provided.
Limitations
Not provided.
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
GPI‑linked antigens: CD59 on RBCs; FLAER on granulocytes and monocytes
Other
Result Turnaround Time
2-3 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
5 mL (3 mL minimum)
Minimum Volume
3 mL
Container
sodium heparin (green‑top), ACD A (yellow‑top) or EDTA (lavender‑top) tube
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Sodium heparin and ACD: 48 hours; EDTA: 30 hours |
Other tests from different labs that may be relevant