Respiratory Syncytial Virus (RSV), Antigen, DFA
Also known as: RSV Slide Test, RSV Direct Antigen Detection
Use
Detection of Respiratory Syncytial Virus antigen via direct fluorescent antibody (DFA) aids in diagnosing active RSV infection, especially in early stages when viral shedding is high, and assists in patient management and infection control decisions.
Special Instructions
Not provided.
Limitations
Negative results do not rule out infection; optimal diagnostic value is during early infection when viral load is highest; specimen transport and handling at improper temperatures or using unacceptable specimen types can compromise the result.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Nasopharyngeal Swab
Volume
3 mL preferred; 1 mL minimum
Minimum Volume
1 mL
Container
Viral transport medium (VCM) or equivalent
Collection Instructions
Place swab or aspirate into VCM; transport refrigerated or on wet ice; if delays, freeze at –70 °C and ship on dry ice; do not ship at –20 °C
Causes for Rejection
Dry swabs; nasal smear; non-respiratory specimens; specimens in formalin or non‑viral transport media; sputum; throat swabs; unpreserved samples
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Unacceptable |
| Refrigerated | 4 days |
| Frozen | 30 days at –70 °C |