Toxoplasma gondii (IgG), ELISA, CSF
Use
Detection of Toxoplasma gondii–specific IgG antibodies in cerebrospinal fluid (CSF) aids in diagnosing central nervous system infections by demonstrating intrathecal antibody production. Interpretation may be confounded by low CSF antibody concentrations, passive transfer from serum, blood–brain barrier integrity, or blood contamination of CSF specimens.
Special Instructions
Specimen must be CSF, collected in sterile container, refrigerated after collection, and shipped under cold chain. Testing days typically Tuesday through Friday.
Limitations
Low sensitivity for CNS toxoplasmosis in some contexts; interpretation affected by passive transfer of serum antibodies and possible contamination via traumatic lumbar puncture.
New York Approved
Methodology
Immunoassay (ELISA)
Biomarkers
Result Turnaround Time
3-10 days
Related Documents
For more information, please review the documents below
Specimen
Cerebrospinal Fluid
Volume
1 mL
Minimum Volume
0.5 mL
Container
Sterile container
Collection Instructions
Submit 1 mL of spinal fluid (CSF) in a sterile, plastic screw‑cap vial. Refrigerate specimen after collection and ship at refrigerate temperature.
Causes for Rejection
Gross hemolysis; gross lipemia; gross icterus; non‑CSF specimens; bacterially contaminated specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 14 days |
| Frozen | 30 days |