Borrelia burgdorferi VlsE1/pepC10 Antibodies, Total by ELISA With Reflex to IgM and IgG by ELISA (Modified Two-Tier Testing)
Also known as: LYME MTTT
Use
The Borrelia burgdorferi VlsE1/pepC10 Antibodies, Total by ELISA is a preferred reflex test to diagnose Lyme disease in symptomatic individuals. It follows the modified two-tier testing (MTTT) approach, where a positive or equivocal screen is confirmed by immunoassay. This process helps in detecting antibodies related to B. burgdorferi, the causative agent of Lyme disease, to provide a dependable diagnosis when symptoms suggest potential exposure to the disease.
Special Instructions
If VlsE1/pepC10 antibodies by ELISA is 0.91 IV or greater, then IgM and IgG by ELISA will be added, and additional charges will apply. Negative results do not require further testing. This test is New York state approved.
Limitations
The test requires careful collection and handling of specimens as CSF or plasma, contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens are unacceptable. There are specific ambient, refrigerated, and frozen stability conditions to preserve specimen integrity. Repeated freeze/thaw cycles may degrade the sample's quality. Negative results do not rule out the presence of Lyme disease, as antibody development may be absent or delayed early in the infection.
FDA ApprovedNew York Approved
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 100711-1
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.15 mL
Container
ARUP Standard Transport Tube
Collection Instructions
Separate serum from cells ASAP or within 2 hours of collection.
Storage Instructions
Refrigerated storage required.
Causes for Rejection
CSF or plasma. Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 2 weeks |
| Frozen | 1 month (avoid repeated freeze/thaw cycles) |
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