Hepatitis B Virus (HBV) Genotype
Also known as: HBV PreCore Mutations, HBV Subtype
Use
The HBV genotype assay classifies the virus present in the sample into one of nine recognized genotypes (A-I) via sequence analysis of the DNA polymerase gene. Disease progression and response to therapy may be influenced by genotype. In addition, the HBV genotype assay identifies and reports sequence polymorphisms in the basal core promotor (BCP) and coding regions of the HBV precore gene that result in either decreased levels, or complete absence, of HBeAg expression in chronically infected patients.
Special Instructions
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Limitations
This assay may not be successful when the HBV viral load is <500 IU/mL. The test has not been cleared or approved by the FDA, but the FDA has determined that such clearance or approval is not necessary.
Methodology
PCR-based (PCR)
Biomarkers
LOINC Codes
- 32366-7
- 54210-0
Result Turnaround Time
7-10 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
1.2 mL
Minimum Volume
600 μL
Container
Gel-barrier tube, yellow-top (ACD) tube, lavender-top (EDTA) tube or PPT
Collection Instructions
Centrifuge sample within six hours of collection, transfer plasma/serum to a polypropylene screw-cap tube and freeze.
Storage Instructions
Freeze.
Causes for Rejection
Quantity not sufficient for analysis; heparin/SPS plasma (wrong anticoagulant); gross hemolysis; specimen not frozen
Other tests from different labs that may be relevant