Hepatitis C Virus (HCV) Genotype 3 NS5A Drug Resistance Assay
Also known as: NS5A Resistance Analysis
Use
Assessment of drug susceptibility by nucleic acid sequencing of a patient's hepatitis C virus (HCV) to the NS5A inhibitors. Assay should be used for patients with documented HCV genotype 3.
Special Instructions
Please confirm the patient's HCV genotype 3 prior to submitting the sample for testing. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Limitations
This procedure may not be successful when the HCV viral load is less than 500 IU/mL. The assay requires an HCV genotype of 3 for successful analysis, and non-compliance with specimen collection and handling instructions may lead to test rejection. Proper centrifugation and freezing of samples are crucial to avoid sample degradation which may lead to erroneous results.
Methodology
NGS (Targeted)
Biomarkers
LOINC Codes
- 72862-6
- 73655-3
- 49549-9
Result Turnaround Time
10-14 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
2 mL
Minimum Volume
1 mL
Container
Ship in plasma preparation tubes (PPT™) or screw-capped polypropylene frozen transport tubes.
Collection Instructions
Collect specimen in two PPT™ tubes, gel-barrier tubes, or lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection, remove serum/plasma, transfer specimen to polypropylene screw-capped tubes, and freeze.
Storage Instructions
Freeze.
Causes for Rejection
PPT™ not centrifuged; insufficient volume; heparinized plasma; nonfrozen specimens; specimens exposed to repeated freeze/thaw cycles; specimen received in 'pop-top' or 'snap-cap' tube.
Other tests from different labs that may be relevant