Hepatitis Delta Virus by Quantitative PCR

Casandra

Casandra Test Code AR12369Version 1 (DRAFT)

Performing Lab

Lab ARUP LaboratoriesLab Test ID 2013881CPT Code 87523

Hepatitis Delta Virus by Quantitative PCR

Also known as: HDV QNT

Clinical Use

Order Test

Use

This test is designed to confirm and quantify the presence of hepatitis D virus (HDV) in serum samples. It is useful for assessing the viral load in patients known to be infected with HDV, which is critical for disease management and monitoring the effectiveness of antiviral therapy. The test uses quantitative polymerase chain reaction (PCR) to measure HDV RNA levels, providing a precise quantification of viral burden. Accurate measurement of viral load aids physicians in tailoring treatment regimens and monitoring disease progression.

Special Instructions

Specimen source is required for testing. Ensure that serum is separated from cells and transported in a sterile container. Maintain specimen stability by keeping it frozen during transport to preserve sample integrity, as ambient and refrigerated stability are limited.

Limitations

The test has a quantitative range from 2.0 to 6.7 log IU/mL (92 to 4,600,000 IU/mL). Results less than 2.0 log IU/mL or 92 IU/mL are considered negative, but may not rule out the presence of PCR inhibitors or HDV RNA concentrations below the detection threshold. Inhibition in samples could lead to underestimation of viral quantitation, potentially affecting the accuracy of the results. Additionally, only HDV genotype 1 was validated through wet bench studies, while other genotypes were validated in silico, limiting the study accuracy for those variants.

Test Details

New York Approved