HER‑2 IHC with Reflex to FISH
Also known as: HER2, Metastatic Cancer, Breast Carcinoma, Gastro-esophageal adenocarcinoma, Immunohistochemical Stain
Use
Determines HER‑2/neu protein overexpression on formalin‑fixed, paraffin‑embedded tumor tissue via immunohistochemistry. Equivocal (2+) results are reflexed to HER‑2 gene amplification assessment by fluorescence in situ hybridization for confirmation, which can guide eligibility for HER‑2‑targeted therapies.
Special Instructions
Reflex to FISH is performed only if IHC result is equivocal (2+) and incurs additional charge.
Limitations
Reliability depends on proper fixation: tissue must be fixed in 10% neutral buffered formalin for 6–72 hours and submitted with accompanying pathology report. Non‑invasive tumors are rejected.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
Not provided.
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Formalin‑fixed paraffin‑embedded tissue block or five unstained (4‑micron) charged slides
Collection Instructions
Submit formalin‑fixed paraffin‑embedded tumor tissue block or five 4‑micron unstained sections on Poly‑L‑Lysine or Silane‑coated slides; do not place paper labels with adhesive backing on slides; use pencil or xylene‑resistant pen to write on the frosted end only
Causes for Rejection
Non‑invasive tumors
Other tests from different labs that may be relevant