HER2 by IHC, Colorectal, With Reflex to FISH if Equivocal
Use
HER2 is a known negative predictive biomarker in colorectal cancer (CRC). Recent trials have established HER2 as a therapeutic target in CRC. This assay assists in determining eligibility for HER2 therapy in patients with CRC.
Special Instructions
Testing will be delayed if the pathology report is not received. Fixation time and fixative used must be indicated on the test requisition form.
Limitations
Use of fixatives other than 10% formalin may not yield reliable results.
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 18474-7
- 18474-7
- 77202-0
- 72486-4
Result Turnaround Time
5 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
One paraffin block or seven unstained, positively charged tissue slides cut at 4-5 microns
Minimum Volume
Five unstained slides
Container
Paraffin block transport pouch or slide mailer
Collection Instructions
Specimen should be fixed in a 10% neutral-buffered formalin. Cold ischemic time should be less than one hour and fixation time should be between six and 72 hours.
Storage Instructions
Maintain specimen at room temperature.
Causes for Rejection
Tumor type other than colorectal cancer (CRC); fixative other than neutral buffered formalin; insufficient tumor cellularity
Other tests from different labs that may be relevant