HLA Class I Antibody and HLA Class II Antibody Screen
Also known as: HLA Antibody, PRA, HLA Class 1/2 Antibody, White Cell Antibody
Use
Solid organ transplantation
Special Instructions
Collect specimen prior to dialysis. Do not collect if the patient has received antithymocyte globulin, OKT3, or alemtuzumab (Campath®) recently, as these drugs may cause false-positive results.
Limitations
The test detects only antibodies of the IgG isotype and does not detect IgM antibodies, which are usually indicative of autoantibodies. This limits its ability to provide detailed information on the specificities of the antibodies against HLA molecules. Additionally, the results should be interpreted with caution as they may not provide a complete picture of the immunological risk related to solid organ transplantation.
Methodology
Other
Biomarkers
LOINC Codes
- 50968-7
- 49549-9
Result Turnaround Time
3-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
7 mL
Minimum Volume
3 mL
Container
Red-top tube or gel-barrier tube
Collection Instructions
To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Storage Instructions
Maintain specimen at room temperature. Protect from extreme heat or cold. Serum may be frozen.
Causes for Rejection
Highly lipemic or hemolyzed specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 2 days |
| Refrigerated | 1 month |
| Frozen | 2 years |
Other tests from different labs that may be relevant