HLA Class II Antibody Identification
Use
Solid organ transplantation
Special Instructions
It is advised to collect the specimen prior to dialysis. Do not collect if the patient has recently received antithymocyte globulin, OKT3, or alemtuzumab (Campath®), as these drugs may lead to false-positive results. Separate frozen specimens should be submitted for each test requested to avoid delays in turnaround time.
Limitations
The test is capable of detecting antibodies of the IgG isotype but does not detect IgM antibodies, which are often indicative of autoantibodies. False positives may occur if the patient has received certain drugs.
Methodology
Other
Biomarkers
LOINC Codes
- 46995-7
- 49549-9
Result Turnaround Time
2-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
7 mL
Minimum Volume
3 mL
Container
Red-top tube or gel-barrier tube
Collection Instructions
To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Patient Preparation
Collect prior to dialysis; do not collect if certain immunosuppressive drugs have been administered recently.
Storage Instructions
Maintain specimen at room temperature. Protect from extreme heat or cold. Serum may be frozen.
Causes for Rejection
Highly lipemic or hemolyzed specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 2 days |
| Refrigerated | 1 month |
| Frozen | 2 years |
Other tests from different labs that may be relevant