Human Immunodeficiency Virus Types 1 and 2 (HIV-1/2) Antibody Differentiation, Supplemental

Casandra

Casandra Test Code AR95153Version 1 (DRAFT)

Performing Lab

Lab ARUP LaboratoriesLab Test ID 2013107CPT Codes 86701, 86702

Human Immunodeficiency Virus Types 1 and 2 (HIV-1/2) Antibody Differentiation, Supplemental

Also known as: HIV AB SUP

Clinical Use

Order Test

Use

This HIV-1/2 antibody differentiation test is used to differentiate between HIV-1 and HIV-2 antibodies in patients who have tested repeatedly reactive on third- or fourth-generation HIV screening tests. It aids in the diagnosis of HIV by discerning which type of HIV antibody, if any, is present. This is critical for proper treatment and management of HIV infection as the management strategies and treatment regimens differ for HIV-1 and HIV-2.

Special Instructions

The test is only to be used when the patient has had a repeatedly reactive result on a third- or fourth-generation HIV screening test. The assay should not be used for blood donor screening, associated reentry protocols, or for screening human cells, tissues, and cellular- and tissue-based products (HCT/P).

Limitations

This test should not be used for blood donor screening or component reentry testing. Additionally, it does not reflex to a nucleic acid test if the results are negative or indeterminate. It is intended for use within a specific multitest algorithm recommended by the Centers for Disease Control and Prevention (CDC) and the Clinical Laboratory Standards Institute (CLSI) for the diagnosis of HIV.

Test Details

FDA Approved

New York Approved

Methodology

Immunoassay (Qualitative Immunoassay)

Biomarkers

HIV-1 Antibody

Protein

HIV-1 Antibody • Other • Categorical (e.g., Positive / Negative / Indeterminate)

HIV-2 Antibody

Protein

HIV-2 Antibody • Other • Categorical (e.g., Positive / Negative / Indeterminate)

LOINC Codes

Order Codes

77202-0

Result Codes

29893-5
30361-0
80203-3

Result Turnaround Time

1-2 days

Specimen

Serum

Volume

1 mL

Minimum Volume

0.5 mL

Container

ARUP standard transport tube dedicated only for HIV testing

Collection Instructions

Collect using serum separator tube (SST), lavender EDTA tube, or pink K2EDTA tube. Separate from cells ASAP or within 2 hours of collection.

Storage Instructions

Frozen storage is required. Stability is 48 hours at ambient temperature, 7 days refrigerated, and up to 8 months frozen without repeated freeze/thaw cycles.