Protein Electrophoresis, with Total Protein and Reflex to IFE, Serum (10269)
Also known as: Electrophoresis, IFE, SPE with Reflex IFE, SPEP
Use
This assay performs serum protein electrophoresis to quantify total protein and detect abnormal protein banding; if abnormal bands are detected, immunofixation electrophoresis (IFE) is reflexively performed to characterize monoclonal or abnormal proteins, aiding in the evaluation of disorders such as multiple myeloma, immunoglobulinopathies, and other dysproteinemias.
Special Instructions
Reflex immunofixation is performed only if abnormal banding is detected. Overnight fasting is preferred for specimen collection.
Limitations
Grossly hemolyzed, lipemic, icteric specimens, or plasma (rather than serum), are rejected. Interpretation may be limited in low‑volume or inadequate samples. Reflex testing incurs additional charges.
Methodology
Other
Biomarkers
Result Turnaround Time
2-7 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
4‑8 mL blood; 4 mL serum required
Minimum Volume
2 mL serum
Container
Red Top (No Additive)
Collection Instructions
Overnight fasting is preferred
Patient Preparation
Overnight fasting is preferred
Causes for Rejection
Gross hemolysis; grossly lipemic; grossly icteric; plasma
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 4 days |
| Refrigerated | 7 days |
| Frozen | 28 days |
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