Pseudocholinesterase, Dibucaine Inhibition
Also known as: PCHE PHENO
Use
Order to detect increased sensitivity in individuals who experience prolonged paralysis following succinylcholine or mivacurium administration. The dibucaine number (DN) is the percent of pseudocholinesterase enzyme activity inhibited by dibucaine. Together, DN and PChE enzyme activity results can help identify individuals at risk for prolongated paralysis post-succinylcholine administration. Decreased PChE activity with DN = 80, alongside decreased PChE, suggests variable risk due to possible organophosphate exposure, liver disease, pregnancy, or succinylcholine presence.
Special Instructions
Not provided.
Limitations
Decreased PChE activity combined with DN >= 80 may be influenced by organophosphate exposure, liver disease, pregnancy, or circulating succinylcholine. Specimen collection should occur 48 hours post-succinylcholine administration.
New York Approved
Methodology
Automated Analyzer (Clinical Chemistry)
Biomarkers
Dibucaine Number
AnalytePseudocholinesterase, Total
Analyte
LOINC Codes
- 2098-2 - Cholinesterase SerPl-cCnc
- 39354-6 - Cholinesterase dibucaine/CHe CFr SerPl
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.25 mL
Container
Serum separator tube, plain red, green (sodium or lithium heparin), lavender (EDTA), or pink (K2EDTA).
Collection Instructions
Allow specimen to clot completely at room temperature. Separate serum or plasma from cells ASAP or within 2 hours.
Patient Preparation
Specimen must be drawn prior to surgery or more than two days following surgery. Do not draw in recovery room.
Storage Instructions
Refrigerate specimen after collection.
Causes for Rejection
Lt. blue (sodium citrate) or gray (oxalate/fluoride). Whole blood.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 4 hours |
| Refrigerated | 1 week |
| Frozen | 3 months |
Other tests from different labs that may be relevant