Rheumatoid Factor, IgG by EIA (RDL)
Use
EIA methods have the added advantage of being able to simultaneously detect RF of IgG and IgA subclasses in addition to RF IgM and are not susceptible to prozone. It has become apparent that the specificity and predictive value of the RF test is substantially increased by the detection of all three RF isotypes.
Special Instructions
Separate serum from cells within one hour and transfer to a plastic transport tube before freezing. Ensure specimens are stored frozen or refrigerated for optimal stability. Avoid grossly hemolyzed, bacterial contamination, lipemic, icteric, and non-serum specimens which may lead to rejection.
Limitations
The test is not susceptible to the prozone effect common in other methods, which makes it reliable for detecting RF of IgG and IgA subclasses alongside RF IgM. However, results should be considered in conjunction with other clinical findings and tests. Variability in test conditions or improper handling can affect results.
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 33314-6
- 33314-6
Result Turnaround Time
7-10 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.3 mL
Container
Red-top tube or gel-barrier tube
Collection Instructions
Separate serum from cells within one hour. Transfer to a plastic transport tube before freezing.
Storage Instructions
Store frozen or refrigerated.
Causes for Rejection
Grossly hemolyzed; bacterial contamination; lipemic, icteric, non-serum specimen types
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 14 days |
| Frozen | 60 days |
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