Sequential 2

Casandra

Casandra Test Code LC55998Version 1 (DRAFT)

Performing Lab

Lab LabcorpLab Test ID 017750CPT Codes 81511, 82105, 82677, 84702, 86336

Also known as: AFP, Maternal, Down Syndrome

Clinical Use

Order Test

Use

Screening test for open neural tube defects, Down syndrome, and trisomy 18

Special Instructions

For accurate testing, the patient must have submitted a previous specimen in the first trimester for the Sequential 1 test. The gestational age is determined using the crown rump length provided with the first trimester specimen. The Maternal Prenatal Screening test request form (0900) should accompany the test. Testing is specifically for the period between 15.0 to 21.9 weeks of gestation.

Limitations

The Sequential 2 test can only be conducted if a specimen was provided in the first trimester under the Sequential 1 protocol. Results from this test are indicative and not diagnostic, meaning a positive screen indicates the need for further diagnostic testing to confirm neural tube defects or chromosomal abnormalities. The test is developed by LabCorp and is not cleared or approved by the FDA.

Test Details

Methodology

Immunoassay (CLIA)

Biomarkers

5 targets

AFPDIAhCGPAPP-AuE3

LOINC Codes

Order Codes

29463-7
43993-5
21484-1
44877-9
11878-6
12146-7
49035-9
48407-1
76348-2
1834-1
23811-3
19080-1
32166-1
2250-9
21264-7
23883-2
36904-1
48803-1
43995-0
49090-4
43994-3
47223-3
49586-1

Result Turnaround Time

2-5 days

Specimen

Serum

Volume

5 mL

Minimum Volume

3 mL

Container

Gel-barrier tube

Collection Instructions

Collect in serum separator tube with gel barrier. Allow blood to clot, avoiding hemolysis. Separate serum from cells by centrifugation. Transport spun tube to testing laboratory. Pour-off is not advised. Maternal serum specimens must be drawn prior to amniocentesis to avoid contamination with fetal blood.