Borrelia burgdorferi Antibodies, IgG and IgM by Immunoblot (CSF)
Also known as: LYME WBCSF
Use
The detection of antibodies to Borrelia burgdorferi in cerebrospinal fluid may indicate a central nervous system infection, suggesting the presence of Lyme neuroborreliosis. This qualitative immunoblot test differentiates between IgG and IgM antibodies to provide further diagnostic context. It is part of the workup for suspected Lyme neuroborreliosis but should not be used in isolation or without consideration of concurrent serum antibody testing and clinical symptoms.
Special Instructions
Not provided.
Limitations
A negative result indicates the absence of antibodies unique to Borrelia burgdorferi, which is not suggestive of Lyme disease. Positive results should be correlated with clinical findings, considering potential contamination by blood or transfer of serum antibodies across the blood-brain barrier. IgM immunoblot is not recommended in chronic stages of Lyme disease. This is a laboratory-developed test and has not been cleared by the FDA.
Methodology
Immunoassay (Western Blot)
Biomarkers
LOINC Codes
- 13203-5 - B burgdor IgM CSF Ql IB
- 13202-7 - B burgdor IgG CSF Ql IB
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Cerebrospinal Fluid
Volume
3 mL
Minimum Volume
2 mL
Container
ARUP Standard Transport Tube
Storage Instructions
Refrigerated
Causes for Rejection
Bacterially contaminated, heat-inactivated, hemolyzed, or xanthochromic specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 8 hours |
| Refrigerated | 2 weeks |
| Frozen | 1 year |
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