Hemoglobin (Hb) A2 and F by Column with Reflex to Electrophoresis
Also known as: HB A2F COL
Use
The test is used to quantify HbA2 and HbF in whole blood, aiding in the management of sickle cell disease and identifying β thalassemia carriers. It provides critical data in laboratory testing to confirm a diagnosis of a β-thalassemia trait by assessing Hb A2 levels in conjunction with family history and additional laboratory data including iron studies and red blood cell indices.
Special Instructions
Not provided.
Limitations
Patients with a combination of iron deficiency and β-thalassemia may have a normal A2 level. An elevated A2 level cannot be used to screen for β-thalassemia in such cases. Other conditions like alpha-thalassemia trait and rare Greek beta-thalassemia variant may not be identified by this assay.
FDA ApprovedNew York Approved
Methodology
Other
Biomarkers
Hemoglobin A2
AnalyteHemoglobin F
Analyte
LOINC Codes
- 13514-5 - Hgb Fract Bld Elph-Imp
- 4551-8 - Hgb A2 MFr Bld
- 4576-5 - Hgb F MFr Bld
- 21026-0 - Path Interp Bld-Imp
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
5 mL
Minimum Volume
0.2 mL
Container
Lavender (EDTA) or pink (K2EDTA)
Storage Instructions
Refrigerated
Causes for Rejection
Frozen or room temperature specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Unacceptable |
| Refrigerated | 1 week |
| Frozen | Unacceptable |
Other tests from different labs that may be relevant