Hemoglobin S, Sickle Solubility
Also known as: SICKLE
Use
The Hemoglobin S, Sickle Solubility test is a qualitative, confirmatory screening test designed to detect the presence of Hemoglobin S (Hb S). It is effective for secondary confirmation of hemoglobin S. However, the test does not differentiate between hemoglobin S trait and homozygous sickle cell disease or other hemoglobin combinations such as S/C, S/D, S/G, S/E, S/thalassemia, S/O-Arab, S/New York, and C-Georgetown trait (Hb C-Harlem). This test is recommended for screening purposes and should be confirmed with further hemoglobin evaluations through electrophoresis in cases where sickle cell disease is suspected.
Special Instructions
This test is not recommended for newborns under 6 months due to high concentrations of fetal hemoglobin (Hb F), which can interfere with results. Confirm positive results with additional hemoglobin testing such as electrophoresis. New York state approved.
Limitations
False negative results can occur if there is anemia, less than 20% of Hb S present due to age in neonates, or after recent transfusion. Elevated Hb F levels can also result in false negatives. False positives may be caused by conditions such as hypergammaglobulinemia, erythrocytosis, extreme leukocytosis, hyperlipidemia, recent transfusions, or high levels of nucleated RBCs. Certain rare sickling hemoglobins like Hb C-Harlem can also produce positive results. The test should be followed by electrophoresis for detailed hemoglobin analysis.
New York Approved
Methodology
Other
Biomarkers
LOINC Codes
- 6864-3
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
5 mL
Minimum Volume
0.2 mL
Container
Lavender (EDTA) or pink (K2EDTA)
Storage Instructions
Refrigerated.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Unacceptable |
| Refrigerated | 1 week |
| Frozen | Unacceptable |