Hemoglobin, Unstable
Also known as: HGB UNSTAB
Use
Unstable hemoglobin disease is caused by alterations in the amino acid sequence at the alpha and beta chain interface of the hemoglobin molecule, leading to structural changes, denaturation, and precipitation of globin chains. This test is used to confirm the unstable nature of hemoglobins, but it does not identify the specific variant present. Additional evaluation is suggested for positive tests, including hemoglobin evaluation by HPLC with reflex to electrophoresis and/or RBC solubility.
Special Instructions
This test cannot be used on infants younger than 6 months due to physiologically elevated Hb F levels. Test results are unreliable in such cases.
Limitations
The Unstable Hemoglobin assay is a qualitative test that does not identify the specific hemoglobin variant. False positive results can occur in specimens with high hemoglobin F levels, such as in infants under 6 months of age. Specimens tested beyond 7 days from draw time may also result in false positives.
New York Approved
Methodology
Other
Biomarkers
LOINC Codes
- 4639-1
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
5 mL
Minimum Volume
2 mL
Container
Lavender (EDTA) or pink (K2EDTA)
Collection Instructions
Transport 5 mL whole blood in lavender (EDTA) or pink (K2EDTA) tube. Do not freeze.
Storage Instructions
Refrigerate specimen during transport. Do not freeze.
Causes for Rejection
Samples more than 7 days old; specimens collected on infants less than 6 months old.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Unacceptable |
| Refrigerated | 7 days |
| Frozen | Unacceptable |