Hepatitis E Virus by Quantitative PCR
Also known as: HEV QNT
Use
This test is used to confirm and quantify the presence of the hepatitis E virus in a patient's specimen. It aids in monitoring viral load and assessing the infection status of individuals suspected of having hepatitis E. The quantitative results can assist clinicians in determining the severity of the infection and in making treatment decisions for managing hepatitis E, particularly important in monitoring immunocompromised patients or those with chronic liver disease.
Special Instructions
This test is New York state approved and must be performed in a CLIA certified laboratory. Specimens should be collected using Lavender (EDTA), pink (K2EDTA), or Serum separator tubes (SST), with serum or plasma being separated from cells before transfer to ARUP Standard Transport Tube. Additional information regarding specimen source should be provided.
Limitations
A negative result does not rule out the presence of PCR inhibitors in the patient specimen or HEV RNA concentrations below the level of detection of the test. Inhibition may lead to an underestimation of viral quantitation. The test's quantitative range is 3.3-8.3 log IU/mL, with one IU/mL equating to approximately 2.25 copies/mL. The test is a Laboratory Developed Test (LDT) and is not FDA cleared or approved.
New York Approved
Methodology
PCR-based (qPCR)
Biomarkers
LOINC Codes
- 31208-2
- 69961-1
- 78750-7
- 90460-7
Result Turnaround Time
2-5 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
1 mL
Minimum Volume
0.5 mL
Container
ARUP Standard Transport Tube
Collection Instructions
Separate from cells before transfer.
Storage Instructions
Frozen
Causes for Rejection
Heparinized specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 1 week |
| Frozen | 1 year |