Human Metapneumovirus by PCR
Also known as: HMPVPCR
Use
This test is used to detect human metapneumovirus (hMPV) in children and immunocompromised adults. It is particularly useful when there is a suspicion of hMPV infection despite a negative result from a direct fluorescent antibody (DFA) test. The test helps in confirming or excluding hMPV as the causative agent of respiratory illness in these populations.
Special Instructions
Not provided.
Limitations
A negative result does not completely rule out the presence of hMPV infection, as PCR inhibitors in the sample or insufficient nucleic acid concentrations could lead to a falsely negative result. The test is qualitative, and assay performance can vary depending on the source specimen and methodological conditions.
Methodology
PCR-based (PCR)
Biomarkers
LOINC Codes
- 31208-2 - Specimen source
- 38917-1 - hMPV RNA Spec Ql NAA+probe
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Nasopharyngeal Swab
Volume
1 mL
Minimum Volume
0.5 mL
Container
Sterile container or viral transport media (ARUP supply #12884)
Collection Instructions
Place nasopharyngeal swabs in viral transport media. Each specimen should be placed in an individually sealed bag.
Storage Instructions
Store frozen for optimal stability; refrigerated specimens are stable for 4 days.
Causes for Rejection
Specimen source not provided.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Unacceptable |
| Refrigerated | 4 days |
| Frozen | 1 month |
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