Human Metapneumovirus by PCR
Also known as: HMPVPCR
Use
This test is used to detect human metapneumovirus (hMPV) in children and immunocompromised adults. It is particularly useful when there is a suspicion of hMPV infection despite a negative result from a direct fluorescent antibody (DFA) test. The test helps in confirming or excluding hMPV as the causative agent of respiratory illness in these populations.
Special Instructions
Specimen source must be provided. Acceptable specimens include nasopharyngeal swabs, bronchoalveolar lavage (BAL), or pleural fluid. Specimens should be transferred to viral transport media and stored frozen for optimal stability.
Limitations
A negative result does not completely rule out the presence of hMPV infection, as PCR inhibitors in the sample or insufficient nucleic acid concentrations could lead to a falsely negative result. The test is qualitative, and assay performance can vary depending on the source specimen and methodological conditions.
New York Approved
Methodology
PCR-based (PCR)
Biomarkers
LOINC Codes
- 31208-2
- 38917-1
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Nasopharyngeal Swab
Volume
1 mL
Minimum Volume
0.5 mL
Container
Sterile container or viral transport media (ARUP supply #12884)
Collection Instructions
Place nasopharyngeal swabs in viral transport media. Each specimen should be placed in an individually sealed bag.
Storage Instructions
Store frozen for optimal stability; refrigerated specimens are stable for 4 days.
Causes for Rejection
Specimen source not provided.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Unacceptable |
| Refrigerated | 4 days |
| Frozen | 1 month |