Human Metapneumovirus DFA
Also known as: HMPVFA
Use
This test is used for the preferred initial detection of human metapneumovirus (hMPV) in both children and adults. Human metapneumovirus is a significant cause of respiratory infections and this assay helps in the prompt identification and management of the infection.
Special Instructions
The specimen source is preferred, and refrigerated transport of the specimen is advised. Specimen collection should be done with care to ensure adequate sample collection as the sensitivity of the DFA test depends on the number of cells present.
Limitations
The sensitivity of the Direct Fluorescent Antibody (DFA) methodology is highly dependent on the adequacy of the specimen. If there are fewer than 20 cells, the DFA result will be reported as 'sample inadequate'. Additionally, certain types of swabs such as calcium alginate, eSwabs, dry, or wood swabs as well as slides are unacceptable for the test.
New York Approved
Methodology
Immunoassay (Immunofluorescent Stain)
Biomarkers
LOINC Codes
- 40979-7
- 66746-9
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Sputum
Volume
3 mL
Minimum Volume
0.5 mL
Container
Sterile container or 3 mL viral transport media
Storage Instructions
Refrigerate specimen.
Causes for Rejection
Calcium alginate, eSwab, dry, or wood swabs. Slides.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 2 hours |
| Refrigerated | 72 hours |
| Frozen | Unacceptable |