IDH1 R132H by Immunohistochemistry
Also known as: IDH1 IHC
Use
The IDH1 R132H by Immunohistochemistry test is used to detect an IDH1 mutation at the R132H site in formalin-fixed, paraffin-embedded tissue specimens. This mutation is present in various types of gliomas and helps in differentiating between diffuse gliomas that harbor the mutation and those that do not. The presence of this mutation is associated with a better prognosis and may guide therapeutic decisions. It is intended for clinical purposes and provides valuable information for the diagnostic workup of brain tumors.
Special Instructions
To order the test, electronic request submission is preferred. If electronic ordering is unavailable, the ARUP Immunohistochemistry Stain Form (#32978) with an ARUP client number must be used. Tissue or cell specimens must be formalin-fixed and paraffin-embedded. If sending precut slides, avoid oven baking, and ensure secure transport.
Limitations
This test has been developed and characteristics determined by ARUP Laboratories as a Laboratory Developed Test (LDT), but it has not been cleared or approved by the FDA. The test is performed as a technical-only service, meaning it only applies the stain and does not provide interpretive pathology services. Specimens with non-representative tissue type or depleted specimens are unacceptable.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 94736-6
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
2 slides
Container
positively charged slides in a tissue transport kit (ARUP supply #47808)
Collection Instructions
Collect tissue or cells and formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen. Protect paraffin block or slides from excessive heat.
Storage Instructions
Room temperature or refrigerated. Ship in cooled container during summer months.
Causes for Rejection
Specimens submitted with non-representative tissue type. Depleted specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |