IRF4/DUSP22 (6p25) Gene Rearrangement by FISH
Also known as: IRF4 FISH
Use
The IRF4/DUSP22 (6p25) Gene Rearrangement by FISH test is designed to detect IRF4 and DUSP22 rearrangements, which are relevant for the diagnosis and prognosis of B-cell and T-cell lymphomas. This assay is crucial for identifying translocations involving chromosome region 6p25, a known marker for non-Hodgkin lymphomas. The test employs fluorescence in situ hybridization (FISH) to analyze paraffin-embedded tissue sections, selecting areas for analysis through histopathologic review.
Special Instructions
If multiple specimens (blocks or slides) are sent to ARUP Laboratories for the same patient, they must be accompanied by an instruction indicating a preferred specimen or individual orders for each sample. A Pathologist Block Selection Fee will apply if the laboratory is required to choose the best specimen without specific instructions.
Limitations
The test is expected to detect 100% of IRF4 rearrangements except for very rare instances of cryptic rearrangements. Approximately 12% or more cells showing abnormal signal patterns indicate a positive result, although other non-classical abnormal patterns might also be present. Results may be affected by the quality of the specimen and should be correlated with morphologic, clinical, and immunophenotypic data to ensure accurate interpretation. The test uses fluorescent probes that target upstream and downstream regions flanking IRF4/DUSP22 genes, ensuring specificity but limiting its use to prepared tumor tissue sections.
New York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
LOINC Codes
- 81304-8
- 94736-6
- 31208-2
- 48674-6
- 19076-9
Result Turnaround Time
3-7 days
Related Documents
For more information, please review the documents below
Specimen
Tumor Tissue (FFPE)
Volume
Not provided
Minimum Volume
4 slides
Container
Positively charged glass slides or tissue block
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed tumor tissue. Transport tissue block or 4 unstained, consecutively cut, 5-micron thick sections, mounted on positively charged glass slides.
Storage Instructions
Protect paraffin block and/or slides from excessive heat.
Causes for Rejection
Specimens fixed in any fixative other than 10 percent neutral buffered formalin, decalcified specimens, or paraffin block with no tumor tissue remaining.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |