Plasminogen Activator Inhibitor 1, Activity
Also known as: PAI-1
Use
This test is designed to detect elevated concentrations of plasminogen activator inhibitor 1 (PAI-1), which is thought to be a thrombotic risk factor. It helps in assessing the risk of thrombosis by measuring PAI-1 activity levels. However, the test is not a first-line diagnostic tool for inherited thrombotic or bleeding disorders. The assay assists in identifying the diurnal variation of PAI-1, with higher concentrations noted in the morning and decreased levels in the afternoon.
Special Instructions
Patient preparation involves specimen collection between 8 a.m. and 12 p.m. Centrifuge plasma and within 1 hour of draw, transfer 1.5 mL platelet-poor plasma to an ARUP Standard Transport Tube and freeze (Min: 1 mL). Adherence to specimen handling guidelines and storage instructions is critical for accurate results.
Limitations
Low concentrations of PAI-1 may not be accurately quantified by this assay, with concentrations below 2.0 IU/mL not being diagnostic of PAI-1 deficiency. The test result of less than 2.0 IU/mL may also be seen in normal individuals. Furthermore, the assay's accuracy is reliant on fasting samples and collection timing due to PAI-1's diurnal variation.
New York Approved
Methodology
Immunoassay (Bioimmunoassay)
Biomarkers
LOINC Codes
- 5975-8
Result Turnaround Time
1-8 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
1.5 mL
Minimum Volume
1 mL
Container
ARUP Standard Transport Tube
Collection Instructions
Centrifuge plasma within 1 hour of draw. Transfer 1.5 mL platelet-poor plasma to an ARUP Standard Transport Tube and freeze. Min: 1 mL.
Patient Preparation
Collect specimen between 8 a.m. and 12 p.m.
Storage Instructions
CRITICAL FROZEN. Additional specimens must be submitted when multiple tests are ordered.
Causes for Rejection
Serum, EDTA plasma, clotted or hemolyzed specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 1 hour |
| Refrigerated | Unacceptable |
| Frozen | 2 months |