Pyruvic Acid, CSF
Also known as: PYRU CSF
Use
This test is useful for investigating potential disorders related to mitochondrial metabolism. Mitochondrial disorders can lead to an imbalance in energy production and are often associated with symptoms affecting multiple organ systems. The test measures pyruvic acid levels in cerebrospinal fluid (CSF), which can provide important information when assessing patients suspected of having mitochondrial dysfunction. It is particularly powerful when used alongside the CSF lactate test, as the lactate-to-pyruvate ratio provides insights into the oxidation capacity of the cells.
Special Instructions
Patient should be at complete rest before specimen collection. It is important to follow the specific specimen preparation and storage instructions to ensure the integrity of the sample for accurate results. The pyruvate collection tube containing 8 percent (w/v) perchloric acid is available via ARUP eSupply.
Limitations
The test's accuracy is highly dependent on the proper preparation and storage of the specimen. If less than 1 mL of CSF is added to the collection tube, the pH of the supernatant may become too low for testing, which can lead to unreliable results. Additionally, deviations from the storage instructions, like not freezing the sample immediately, can affect test outcome.
New York Approved
Methodology
Automated Analyzer (Clinical Chemistry)
Biomarkers
LOINC Codes
- 14122-6
- 14122-6
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Cerebrospinal Fluid
Volume
1 mL
Minimum Volume
1 mL
Container
pyruvate collection tube containing 2 mL 8 percent (w/v) perchloric acid
Collection Instructions
Immediately after CSF is drawn, add exactly 1 mL CSF to a chilled pyruvate collection tube. Mix well for 30 seconds then place in an ice bath for 10 minutes. Centrifuge for 10 minutes at 1500 x g. Decant 2 mL supernatant to an ARUP Standard Transport Tube and freeze.
Patient Preparation
Patient should be at complete rest.
Storage Instructions
Frozen.
Causes for Rejection
If less than 1 mL of CSF is added to the collection tube, pH of the supernatant will be too low for testing.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Unacceptable |
| Refrigerated | 2 days |
| Frozen | 1 month |