Pyruvic Acid, CSF

Also known as: PYRU CSF

Clinical Use

Use

This test is useful for investigating potential disorders related to mitochondrial metabolism. Mitochondrial disorders can lead to an imbalance in energy production and are often associated with symptoms affecting multiple organ systems. The test measures pyruvic acid levels in cerebrospinal fluid (CSF), which can provide important information when assessing patients suspected of having mitochondrial dysfunction. It is particularly powerful when used alongside the CSF lactate test, as the lactate-to-pyruvate ratio provides insights into the oxidation capacity of the cells.

Special Instructions

Not provided.

Limitations

The test's accuracy is highly dependent on the proper preparation and storage of the specimen. If less than 1 mL of CSF is added to the collection tube, the pH of the supernatant may become too low for testing, which can lead to unreliable results. Additionally, deviations from the storage instructions, like not freezing the sample immediately, can affect test outcome.

Test Details

New York Approved

Methodology

Automated Analyzer (Clinical Chemistry)

Biomarkers

Pyruvic Acid

Analyte

Concentration / Level

Concentration / Level • Quantitative (Continuous) (e.g., copies/mL, % expression)

LOINC Codes

Order Codes

14122-6 - Pyruvate CSF-sCnc

Result Codes

14122-6 - Pyruvate CSF-sCnc

Result Turnaround Time

1-2 days

Specimen

Cerebrospinal Fluid

Volume

1 mL

Minimum Volume

1 mL

Container

pyruvate collection tube containing 2 mL 8 percent (w/v) perchloric acid

Collection Instructions

Immediately after CSF is drawn, add exactly 1 mL CSF to a chilled pyruvate collection tube. Mix well for 30 seconds then place in an ice bath for 10 minutes. Centrifuge for 10 minutes at 1500 x g. Decant 2 mL supernatant to an ARUP Standard Transport Tube and freeze.